Journal: Acta Neuropathologica Communications

Article Title: Murine versus human apolipoprotein E4: differential facilitation of and co-localization in cerebral amyloid angiopathy and amyloid plaques in APP transgenic mouse models

doi: 10.1186/s40478-015-0250-y

Figure Lengend Snippet: Co-localization of mouse apoE and apoE4 in CAA or plaques within the same brain parenchyma in 5XFAD/apoE m/4 mice. 10-month-old 5XFAD/apoE m/4 mice were co-stained with HJ6.3-Alexa 568 for mouse apoE, HJ15.7-Alexa 488 for apoE4, and X-34 for fibrillar amyloid. a - b Representative images of co-staining for mouse apoE and apoE4 in the plaques and % area of plaque covered by different apoE. c - d Representative images of co-staining for mouse apoE and apoE4 in parenchymal CAA and % area of parenchymal CAA covered by different apoE. Values connected by lines were measured from the same animals ( n = 9/group; * p < 0.05, *** p < 0.001, paired t -test)

Article Snippet: The standard curve was mouse apoE purified from mouse astrocytes conditioned medium using a polyclonal antibody (Calbiochem).

Techniques: Staining

Journal: Acta Neuropathologica Communications

Article Title: Murine versus human apolipoprotein E4: differential facilitation of and co-localization in cerebral amyloid angiopathy and amyloid plaques in APP transgenic mouse models

doi: 10.1186/s40478-015-0250-y

Figure Lengend Snippet: Comparison between apoE co-localization in parenchymal and leptomeningeal CAA in the same 5XFAD/apoE m/4 brains. Brain sections from 10 months old 5XFAD/apoE m/4 animals were co-stained with HJ6.3-Alexa 568 for mouse apoE, HJ15.7-Alexa 488 for human apoE4, and X-34 for fibrillar amyloid. a - b Representative images of co-staining for mouse apoE and apoE4 in the leptomeningeal CAA and % area of leptomeningeal CAA covered by different apoE ( n = 9/group). Scale bars, 50 μm. c % area of plaque, parenchymal and leptomeningeal CAA covered by mouse apoE. d % area of plaque, parenchymal and leptomeningeal CAA covered by human apoE4. Values connected by lines were measured from the same animals ( n = 8/group; ** p < 0.01, *** p < 0.001, One-way ANOVA repeated measures)

Article Snippet: The standard curve was mouse apoE purified from mouse astrocytes conditioned medium using a polyclonal antibody (Calbiochem).

Techniques: Staining

Journal: Acta Neuropathologica Communications

Article Title: Murine versus human apolipoprotein E4: differential facilitation of and co-localization in cerebral amyloid angiopathy and amyloid plaques in APP transgenic mouse models

doi: 10.1186/s40478-015-0250-y

Figure Lengend Snippet: Co-localization of mouse apoE and apoE4 in plaques within the same brain parenchyma in APPPS1-21/apoE m/4 mice. Brain sections from 85-day-old APPPS1-21/apoE m/4 animals ( n = 7) were co-stained with HJ6.3-Alexa 568 for mouse apoE, HJ15.7-Alexa 488 for human apoE4, and X-34 for fibrillar amyloid. a Representative images and the % of plaques containing both mouse apoE and apoE4 (scale bar, 20 μm). b Representative images and the % of plaques containing only mouse apoE (scale bar, 20 μm)

Article Snippet: The standard curve was mouse apoE purified from mouse astrocytes conditioned medium using a polyclonal antibody (Calbiochem).

Techniques: Staining

Journal: Acta Neuropathologica Communications

Article Title: Murine versus human apolipoprotein E4: differential facilitation of and co-localization in cerebral amyloid angiopathy and amyloid plaques in APP transgenic mouse models

doi: 10.1186/s40478-015-0250-y

Figure Lengend Snippet: ApoE levels in 85-day-old apoE m/4 and APPPS1-21/apoE m/4 brains. The cortices were homogenized in PBS, followed by 1 % Triton X-100 and 5 M guanidine and apoE levels were measured by ELISA. a - b Absolute concentrations and fractional distribution of mouse apoE and apoE4 in apoE m/4 mice ( n = 10). c - d Absolute concentrations and fractional distribution of mouse apoE and apoE4 in APPPS1-21/apoE m/4 mice ( n = 10; * p < 0.05; ** p < 0.01; *** p < 0.001, Two-way ANOVA for repeated measures followed by Bonferroni post-test). e - g Correlations of Aβ 42 with mouse apoE, apoE4 and Aβ 40 in the insoluble fraction of APPPS1-21/apoE m/4 cortices ( n = 10)

Article Snippet: The standard curve was mouse apoE purified from mouse astrocytes conditioned medium using a polyclonal antibody (Calbiochem).

Techniques: Enzyme-linked Immunosorbent Assay